1. VirtualBox

1.1. VHD Image

  • Start Windows Explorer.

  • Create the D:\VirtualBox\VirtualBox VMs\Ubuntu 16.04 - Bioinformatics folder.

  • Copy W:\Images\Ubuntu\Training-Clone.vhd to the D:\VirtualBox\VirtualBox VMs\Ubuntu 16.04 - Bioinformatics folder.

  • Close Windows Explorer.

1.2. Create new virtual machine

  • Start VirtualBox.

  • Click New.

    Name:                   Ubuntu 16.04 - Bioinformatics
Type:                   Linux
Version:                Ubuntu (64-bit)

  • Click Next.

    Memory size:            10240 MB

  • Click Next.

  • Choose Use an existing virtual hard disk file.

  • Click the Choose a virtual hard disk file button.

  • Select the D:\VirtualBox\VirtualBox VMs\Ubuntu 16.04 - Bioinformatics folder.

  • Select the Training-Clone.vhd file.

  • Click Open.

  • Click Create.

  • Close VirtualBox.

1.3. Configure new virtual machine

  • Start VirtualBox.

  • Select the Ubuntu 16.04 - Bioinformatics virtual machine.

  • Click Settings.

  • Select the General page.

  • Select the Advanced tab.

  • Select Shared Clipboard > Bidirectional.

  • Select the System page.

  • Select the Motherboard tab.

  • Uncheck Boot Order > Floppy.

  • Check Hardware Clock in UTC Time.

  • Select the Processor tab.

  • Select Processor(s) > 4.

  • Select the Shared Folders page.

  • Click the Adds new shared folder button.

  • Select Folder Path > Other.

  • Select the D:\ folder.

  • Click Select Folder.

  • Check Auto-mount.

    Folder Path:            D:\
Folder Name:            D_DRIVE
                        □ Read-only
                        ■ Auto-mount

  • Click OK.

  • Click OK.

  • Close VirtualBox.

2. Start virtual machine

  • Start VirtualBox.

  • Select the Ubuntu 16.04 - Bioinformatics virtual machine.

  • Click Start.

  • Do your thing.

  • Shutdown the Ubuntu 16.04 - Bioinformatics virtual machine.

  • Close VirtualBox.

3. Configuration

3.1. Default keyboard

  • Log on as Bioinfo Training.

  • Open System Settings > Text Entry.

  • Select English (US).

  • Click the ^ button to make English (US) the first choice.

  • Close Text Entry.

  • Click the En icon and select English (US).

  • Log off.

3.2. Locale

sudo update-locale LANG="en_US.UTF-8" LANGUAGE="en_US"
sudo dpkg-reconfigure locales
# Press Tab key to select "OK" and press Enter.
# Select "en_US.UTF-8".
# Press Tab key to select "OK" and press Enter.

  • Log off.

  • Log on as Bioinfo Training.

  • Check Don’t ask me this again.

  • Click Keep Old Names.

3.3. Updates

Make sure that the host computer has direct internet access.

  • Log on as Bioinfo Training.

  • Start Terminal.

  • Enter the following commands at a Command Line.

    sudo apt-get update
sudo apt-get upgrade --yes
sudo apt-get dist-upgrade --yes
sudo apt-get autoremove --yes

  • Close Terminal.

3.4. GRUB Configuration

Enter the following commands at a Command Line.

sudo cp -a /etc/default/grub /etc/default/grub.org
sudo sed -i 's/GRUB_HIDDEN_TIMEOUT=30/GRUB_HIDDEN_TIMEOUT=5/' /etc/default/grub
sudo update-grub

3.5. Samba Configuration

Make sure that the host computer has direct internet access.

Enter the following commands at a Command Line.

sudo apt-get install cifs-utils --yes
sudo mount -t cifs -o iocharset=utf8,username=<User>,domain=SMRU,vers=2.0 //SMRU-SRV/Teams$ /mnt
dir /mnt
sudo umount /mnt

sudo cp -a /etc/samba/smb.conf /etc/samba/smb.conf.org
sudo sed -i "/^\[global\]/a client max protocol = SMB3" /etc/samba/smb.conf
sudo sed -i "/^\[global\]/a client min protocol = SMB2" /etc/samba/smb.conf

3.6. Screen lock time

  • Open System Settings > Brightness & Lock.

  • Select Turn screen off when inactive for: Never.

  • Disable Lock.

  • Close Brightness & Lock.

3.7. Timezone

  • Open System Settings > Time & Date.

  • Select the Time & Date tab.

  • Select the Bangkok location by clicking on Thailand in the world map.

  • Close Time & Date.

3.8. VirtualBox Guest Additions

  • Select Devices > Insert Guest Additions CD image.

  • Click Cancel in the popup window.

  • Enter the following commands at a Command Line.

    sudo su -
apt-get install dkms --yes
cp -a /etc/group /etc/group.org
cp -a /etc/gshadow /etc/gshadow.org
gpasswd -a training vboxsf
cp -a /media/training/VBox_GAs_5.2.18/VBoxLinuxAdditions.run .
./VBoxLinuxAdditions.run
vdir /opt
exit

  • Select Devices > Optical Drives > Remove disk from virtual drive.

  • Click Force Unmount.

  • Restart the virtual machine.

4. Usage

  • Log on as Bioinfo Training.

  • Start Files.

  • Select Connect to Server.

  • Archive share: Type smb://SMRU-HyperV01/Archive$ for the Server Address.

  • Teams share: Type smb://SMRU-SRV/Teams$ for the Server Address.

  • Click Connect.

  • Choose Registered User.

    Connect As              ○ Anonymous
                        ● Registered User

Username                <User>
Domain                  SMRU
Password                ********

○ Forget password immediately
○ Remember password until you logout
● Remember forever

  • Click Connect.

  • Do your thing.

  • Select the Archive$ on SMRU-HyperV01 > Eject button.

  • Select the Teams$ on SMRU-SRV > Eject button.

  • Close Files.